Júdová, Jana, 1970- Transformation of tobacco plants with cDNA encoding honeybee royal jelly MRJP1 / Jana Júdová ... [et al.]. -- For expression of MRJP1 - the most abundant protein of honeybee royal jelly - in plants, plasmid carrying the expression cassette composed of CaMV 35S RNA promoter, cDNA encoding MRJP1 with its native signal peptide, and nos3' as transcription terminator in binary vector pBin19 was prepared. The plasmid was introduced into tobacco (Nicotiana tabacum L. cv. Wi38) plants by Agrobacterium tumefaciens-mediated transformation. Transgenic F-1 and F-2 generation was grown from the seeds of the primary obtained transgenic tobacco plants. Immunoblot analyses of protein leaf extracts from transgenic plants showed expression of MRJP1.

In Biologia Plantarum. -- Prague : The Czech Academy of Sciences, 2004. -- ISSN 0006-3134. -- ISSN 1573-8264. -- Vol. 48, no. 2 (2004), pp. 185-191

1. Agrobacterium tumefaciens 2. major royal jelly protein 3. Nicotiana tabacum 4. recombinant protein 5. transgenic plant

I. Šutka, R.
II. Klaudíny, Jaroslav
III. Lišková, D.
IV. Ow, D. W.
V. Šimúth, Jozef
VI. Biologia Plantarum. -- Vol. 48, no. 2 (2004), pp. 185-191

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